CellFace aims to establish digital, holographic flow cytometry as a label-free platform technology for cellular diagnostics. We are an interdisciplinary project of TU Munich coordinated by the Chair for Biomedical Electronics and the Chair for Data Processing in cooperation with several departments of the University Hospital Klinikum rechts der Isar.
Blood delivers a variety of insights into health conditions of an organism, especially in the ﬁeld of in-vitro diagnostics. Therefore, hematological analysis such as complete blood count (CBC) represent a high share of laboratory tests in the health care sector. Despite the availability of largely automated analyzers, the gold standard for the routine diagnosis of hematological disorders is the tedious Giemsa stained blood smear  which suﬀers from inter-observer variations. Further drawbacks of current approaches are a high eﬀort for sample preparation, material expenses, a long processing time or missing clinical relevance . To overcome the mentioned issues and specially to reduce time-consuming manual intervention, the CellFace project was created. Flow cytometry in combination with digital holographic microscopy (DHM)  enables researchers to record 3D images of each single cell, while sustaining a high throughput stream of blood cells. The overall goal is to establish this label- and reagent-free approach as a platform technology for automated blood cell diagnosis . The best methodologies to analyze the recorded phase images are subject to research. Therefore, this work will investigate classical and modern techniques in the ﬁeld of computer vision and machine learning on their suitability for interpreting the images and the contained information.
Scientific and Technical Goals
We develop a novel in vitro diagnostic method for cell analysis and biomarkers which are not accessible today for clinical routine. With access to clinical samples, a highly accurate sample presentation and imaging solution, we can offer AI-induced data exploration and interpretations of volatile biomarker panels.
A tiny drop of blood gives us a detailed picture of patient health and lets us support clinicians in their decision-making. Our label-free technology enables us to provide insights in different health aspects varying from cardiovascular risk assessment to leukemia detection and all that within just a few minutes.
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